Jurkat cells were subjected to SDS PAGE followed by western blot with 12868-1-AP (TRAF5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
HeLa cells were subjected to SDS PAGE followed by western blot with 66991-1-Ig (TRAF5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
HeLa cells were subjected to SDS PAGE followed by western blot with 66991-1-Ig (TRAF5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66991-1-PBS in a different storage buffer formulation.
Jurkat cells were subjected to SDS PAGE followed by western blot with 26917-1-AP (TRAF5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.